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gene exp reg3g mm00441127 m1  (Thermo Fisher)
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(A) Simplified diagram of the strategy used to generate Cdhr2 Δepi mice from Cdhr2 fl/fl in combination with tissue-specific expression of Cre; previously described in Pinette et al. 2019 . (B) Diagram of breeding strategy. Using a background of Cdhr2 fl/fl mice, introduction of a single copy of villin-Cre ( villin-Cre +/- ) in the mating pair results in a mixed litter of Cdhr2 fl/fl and Cdhr2 Δepi mice. (C) The expression of Cxcl1, Il22, Il17A, Il6 and <t>Reg3g</t> in RNA extracted from the terminal ileum at 6-8 weeks of age was assessed by qRT-PCR. Relative units are calculated as described in the Materials and Methods, Gapdh was used as endogenous control. Statistical analysis performed using unpaired t-tests. Error bars represent mean ± SEM. (D-G) Flow cytometry analysis of T cells within the lamina propria in knockout C dhr2 1′epi and control Cdhr2 fl/fl mice. (D) Two-dimensional flow cytometry plots show the gating strategy of CD45 + immune cells in gastrointestinal tissue to identify CD3 + T cells within CD45 + immune cells, CD4 + and CD8 + T cells and γδ T cells. (E) Histograms show gating strategy used to identify IL-17A and IFN-γ expressing cells in CD8 + T cells, CD4 + T cells, and γδ T cells. (F) Violin plots show proportions of T cell populations in the lamina propria of matched Cdhr2 fl/fl and Cdhr2 Δepi mice (CD3 + T cells over total CD45 + T cells) and (CD4 + T cells, CD8 + T cells and γδ T cells over total CD3 + T cells). (G) Violin plots show proportions of IL-17A expressing T cells (CD4, CD8), lineage negative and γδ T cells, in the small intestine lamina propria of matched Cdhr2 fl/fl and Cdhr2 Δepi mice, n = 3. Statistical analysis performed using paired Wilcoxon test.
Gene Exp Reg3g Mm00441127 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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reg3g elisa kit  (Cusabio)
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(A) Simplified diagram of the strategy used to generate Cdhr2 Δepi mice from Cdhr2 fl/fl in combination with tissue-specific expression of Cre; previously described in Pinette et al. 2019 . (B) Diagram of breeding strategy. Using a background of Cdhr2 fl/fl mice, introduction of a single copy of villin-Cre ( villin-Cre +/- ) in the mating pair results in a mixed litter of Cdhr2 fl/fl and Cdhr2 Δepi mice. (C) The expression of Cxcl1, Il22, Il17A, Il6 and <t>Reg3g</t> in RNA extracted from the terminal ileum at 6-8 weeks of age was assessed by qRT-PCR. Relative units are calculated as described in the Materials and Methods, Gapdh was used as endogenous control. Statistical analysis performed using unpaired t-tests. Error bars represent mean ± SEM. (D-G) Flow cytometry analysis of T cells within the lamina propria in knockout C dhr2 1′epi and control Cdhr2 fl/fl mice. (D) Two-dimensional flow cytometry plots show the gating strategy of CD45 + immune cells in gastrointestinal tissue to identify CD3 + T cells within CD45 + immune cells, CD4 + and CD8 + T cells and γδ T cells. (E) Histograms show gating strategy used to identify IL-17A and IFN-γ expressing cells in CD8 + T cells, CD4 + T cells, and γδ T cells. (F) Violin plots show proportions of T cell populations in the lamina propria of matched Cdhr2 fl/fl and Cdhr2 Δepi mice (CD3 + T cells over total CD45 + T cells) and (CD4 + T cells, CD8 + T cells and γδ T cells over total CD3 + T cells). (G) Violin plots show proportions of IL-17A expressing T cells (CD4, CD8), lineage negative and γδ T cells, in the small intestine lamina propria of matched Cdhr2 fl/fl and Cdhr2 Δepi mice, n = 3. Statistical analysis performed using paired Wilcoxon test.
Reg3g Elisa Kit, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
reg3g elisa kit - by Bioz Stars, 2026-02
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(A) Simplified diagram of the strategy used to generate Cdhr2 Δepi mice from Cdhr2 fl/fl in combination with tissue-specific expression of Cre; previously described in Pinette et al. 2019 . (B) Diagram of breeding strategy. Using a background of Cdhr2 fl/fl mice, introduction of a single copy of villin-Cre ( villin-Cre +/- ) in the mating pair results in a mixed litter of Cdhr2 fl/fl and Cdhr2 Δepi mice. (C) The expression of Cxcl1, Il22, Il17A, Il6 and Reg3g in RNA extracted from the terminal ileum at 6-8 weeks of age was assessed by qRT-PCR. Relative units are calculated as described in the Materials and Methods, Gapdh was used as endogenous control. Statistical analysis performed using unpaired t-tests. Error bars represent mean ± SEM. (D-G) Flow cytometry analysis of T cells within the lamina propria in knockout C dhr2 1′epi and control Cdhr2 fl/fl mice. (D) Two-dimensional flow cytometry plots show the gating strategy of CD45 + immune cells in gastrointestinal tissue to identify CD3 + T cells within CD45 + immune cells, CD4 + and CD8 + T cells and γδ T cells. (E) Histograms show gating strategy used to identify IL-17A and IFN-γ expressing cells in CD8 + T cells, CD4 + T cells, and γδ T cells. (F) Violin plots show proportions of T cell populations in the lamina propria of matched Cdhr2 fl/fl and Cdhr2 Δepi mice (CD3 + T cells over total CD45 + T cells) and (CD4 + T cells, CD8 + T cells and γδ T cells over total CD3 + T cells). (G) Violin plots show proportions of IL-17A expressing T cells (CD4, CD8), lineage negative and γδ T cells, in the small intestine lamina propria of matched Cdhr2 fl/fl and Cdhr2 Δepi mice, n = 3. Statistical analysis performed using paired Wilcoxon test.

Journal: bioRxiv

Article Title: Brush border intermicrovillar adhesion limits bacteria attachment to the small intestine brush border

doi: 10.1101/2025.11.25.690363

Figure Lengend Snippet: (A) Simplified diagram of the strategy used to generate Cdhr2 Δepi mice from Cdhr2 fl/fl in combination with tissue-specific expression of Cre; previously described in Pinette et al. 2019 . (B) Diagram of breeding strategy. Using a background of Cdhr2 fl/fl mice, introduction of a single copy of villin-Cre ( villin-Cre +/- ) in the mating pair results in a mixed litter of Cdhr2 fl/fl and Cdhr2 Δepi mice. (C) The expression of Cxcl1, Il22, Il17A, Il6 and Reg3g in RNA extracted from the terminal ileum at 6-8 weeks of age was assessed by qRT-PCR. Relative units are calculated as described in the Materials and Methods, Gapdh was used as endogenous control. Statistical analysis performed using unpaired t-tests. Error bars represent mean ± SEM. (D-G) Flow cytometry analysis of T cells within the lamina propria in knockout C dhr2 1′epi and control Cdhr2 fl/fl mice. (D) Two-dimensional flow cytometry plots show the gating strategy of CD45 + immune cells in gastrointestinal tissue to identify CD3 + T cells within CD45 + immune cells, CD4 + and CD8 + T cells and γδ T cells. (E) Histograms show gating strategy used to identify IL-17A and IFN-γ expressing cells in CD8 + T cells, CD4 + T cells, and γδ T cells. (F) Violin plots show proportions of T cell populations in the lamina propria of matched Cdhr2 fl/fl and Cdhr2 Δepi mice (CD3 + T cells over total CD45 + T cells) and (CD4 + T cells, CD8 + T cells and γδ T cells over total CD3 + T cells). (G) Violin plots show proportions of IL-17A expressing T cells (CD4, CD8), lineage negative and γδ T cells, in the small intestine lamina propria of matched Cdhr2 fl/fl and Cdhr2 Δepi mice, n = 3. Statistical analysis performed using paired Wilcoxon test.

Article Snippet: Primer and probe sets were purchased as Taqman Gene Expression Assays from Applied Biosystems [mouse primer sets: Cxcl1 Mm04207460_m1, Il22 Mm01226722_g1, Il6 Mm00446190_m1, Il17a Mm00439618_m1, Reg3g Mm00441127_m1 and Gapdh Mm99999915_g1].

Techniques: Expressing, Quantitative RT-PCR, Control, Flow Cytometry, Knock-Out